AFC Sudbury were beaten 3-0 at Brightlingsea Regent on Easter Monday, a result that sees them slip to fourth place in the Ryman League Division One North.
It came after an excellent 5-1 win at home to Barkingside on Saturday.
AFC stay in the play-off places, but have been overtaken by Brentwood Town, who beat Tilbury 1-0. Brentwood are a point ahead, with a game in hand.
In the Thurlow Nunn League Premier Division, Hadleigh United went down 5-1 at Whitton United. Hadleigh were 5-0 down at half-time, David Lorimer getting their goal late in the second half.
Long Melford, 3-0 winners over Team Braintree, are top of the First Division, thanks to goals from Nathan Rowe, Jamie Griffiths and Steve Adams.
They overtook Swaffham Town, who were beaten 1-0 by promotion challengers Great Yarmouth Town. Melford are a point ahead of Swaffham, and six points ahead of Great Yarmouth, both of whom have a game in hand.
AFC Sudbury Reserves beat Halstead Town 4-1. Wayne Blackman scored two, with Tom Wingfield and Jordan Brazitis getting the others for AFC. Halstead were trailing 3-0 before Lewis Cunliffe replied from the penalty spot.